Title : A Study of the hBCAT Protein in Immortal Cell Lines.
Aims and Hypothesis:
Main Aim 1:
To experimentally determine the resistance of cells containing hBCAT to anticancer therapies such as chemotherapy in terms of the levels of Cytotoxicity and genotoxicity when the cells are subjected to different doses of chemotherapeutic drugs. This will be done by using different cytotoxity assays to test for the cell viability of cells in a cell culture containing hBCAT after the culture has been incubated with different doses of chemotherapeutic drugs.
Main Aim 2:
To investigate the complex biochemical activities of hBCAT and its potential therapeutic significance in the improvement of the clinical outcomes of chemotherapy
Sub Aim 1:
To assess the effects of chemotherapy on the metabolic activity, cell viability and genotoxicity of the cell cultures treated with various chemotherapeutic drugs
It is hypothesized that blocking the expression of hBCAT will result in increased chemotherapy induced cytotoxicity and genotoxicit